“Atomic Force Microcopy-Based Nanoscale Biomechanics”
Dr. Majid Minary, Department of Mechanical Engineering, Northwestern University
Nanotechnology, the understanding and control of matter at sub-100 nm scale, has the potential to transform almost every aspect of our lives by providing enabling solutions to address some of the most pressing problems in health care. It is predicted that nanotechnology will play a leading role in finding cures for many diseases, and provide early stages of diagnostics that cannot be detected with conventional tools. In this seminar, I will discuss three nanotechnology-enabled topics for biomechanical applications. First, I will present an AFM technique based on extended-nanowire probes for high-resolution imaging of the membrane of living cells. I will show that this new technique is capable of imaging the membrane of living cell with interaction forces as small as 60 pN and an intrinsic quality factor of over 100. In the context of nanoscale biomechanics, I will discuss nanomechanical and electromechanical heterogeneity in individual type I collagen fibrils, as small as 50 nm in diameter, and their implications in bone toughness and piezoelectricity at the multiscale. I will then present a newly developed single-cell electroporation system based on fluidic nanofountain probes (NFPs). Using this platform, biomolecules can be specifically delivered to individual cells, in a potentially parallel manner and high viability, opening new ways for high-throughput drug delivery with single cell resolution.
Dr. Majid Minary is currently a Postdoctoral Fellow in the Department of Mechanical Engineering at Northwestern University, where he has developed a probe-based microfluidic platform for single-cell electroporation for biomolecular delivery. He received his PhD degree in Mechanical Engineering from University of Illinois at Urbana-Champaign. He focused on nanoscale biomechanics and invented an AFM-based technique for high-resolution imaging of the membrane of living cells. He obtained his MS degree from the Center for Applied Biomechanics, University of Virginia, and BS degree from Sharif University of Technology in Tehran, Iran. He is the recipient of Mavis Memorial Fund Scholarship Award from University of Illinois. He is author of more than 15 Journal papers and a book chapter, and co-inventor a pending patent, and has been active reviewer for several Journals.